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Cytology Specimens:
Allow the slide preparations to air dry completely. Please do not heat- or alcohol-fix the slides.

Using a pencil, on the frosted end of the slide, identify each slide with the owner's last name or pet's name, and the site (A, B, or C etc). Use a permanent "Sharpie" type marker to identify source for non-frosted glass slides.

Place the slide(s) specimen-side up in the slot(s) of the mailer.

Complete the information on the cytology slide mailer label provided and apply the label to the front of the mailer, folding over the excess to seal the side securely.

Place the sealed mailer inside the specimen compartment of the sample/requisition bag.

Complete the BCE requisition form (purple ink) and RETAIN BOTTOM COPY for your patient record. Fold the requisition in half and insert in the document pouch of the sample bag such that the owner's name and clinic identification are visible through the side.


Biopsy and Single Tissue Specimens for Histopathology:
State and federal regulations prohibit the handling and transport of specimens in containers not approved for formalin solution. Please do not use dispensing vials for mediations, glass or plastic jars/bottles, or other non-approved containers to transport tissue specimens in formalin solution.

Approved specimen containers of varying sizes, pre-filled with 10% neutral buffered formalin are available from PVPS without charge. If needed, please contact client services at (803) or (866) 252-1913 to request containers for histopathology specimens.

As quickly as possible following excision, place the tissue specimen into 10% neutral buffered formalin solution of an appropriately-sized container to maximize quality of tissue integrity.

Complete the information on the biopsy container label provided and apply to the container. If multiple sites/tissues are submitted, place each specimen in a separate container and label site A, B, C, etc. Ensure lids are closed tightly.

Place all containers for a single case in the specimen pouch of the sample bag. Complete the BCE requisition (purple ink) and RETAIN THE BOTTOM COPY for your patient records. Fold the requisition in half, and insert into the document pouch of the sample bag such that the owner's name and clinic identification are visible through the side.


Necropsy Specimens - Formalin-Fixed Tissues:
State and federal regulations prohibit the handling and transport of specimens in containers not approved for formalin solution. Please do not use dispensing vials for mediations, glass or plastic jars/bottles, or other non-approved containers to transport tissue specimens in formalin solution.

Approved specimen containers of larger sizes, pre-filled with 10% neutral buffered formalin are available from PVPS without charge. Please contact client services at (803) or (866) 252-1913 to request containers suitable for necropsy tissues.

Tissue sections should be collected at a thickness no greater than 5-6 mm to maximize diffusion of formalin into the specimen for proper fixation. Sections should include ALL anatomic areas of an organ (for example, kidney should include both cortex and medulla). Multiple tissues may be submitted in the same container. The ratio of formalin to tissue in the specimen container should be AT LEAST 2:1, with all tissues below the surface of the formalin solution.


Necropsy Specimens - Animals:
Animals to be submitted for complete necropsy examination should be held cooled or refrigerated until transported if at all possible. If transport cannot be arranged within 1 day, freezing the body may be preferred. Please call the pathologist to discuss details of cases prior to freezing an animal being submitted for necropsy examination as this process may interfere with diagnosis of some conditions.

Place the body in a thick plastic bag, tying the opening securely. Place this bag inside a second bag and again close the opening securely. Identify the clinic, owner, and pet by name on the outside of the second bag, visible to the courier.

Use the PME requisition (black ink) for submission of formalin-fixed tissues or whole animals.

  • Enter information in all sections of the requisition as completely as possible to facilitate the examination. If submitting fixed tissues indicate by check mark each tissue collected.
  • Indicate the type of examination requested and the method of disposition of the remains.
  • Ensure that an AUTHORIZING SIGNATURE is on the requisition.
  • RETAIN THE BOTTOM COPY of the triplicate requisition for your patient record. Fold the remaining 2 copies of the requisition and place in a small specimen submission bag with the practice information visible. Attach the requisition bag to the outside of the specimen bag.
  • Transport the animal by courier or by private vehicle to the PVPS necropsy facility. See SUBMISSION PROCEDURES FOR NECROPSY EXAMINATIONS for more details.

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